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The plants are kept in dark before determining the factory essential for photosynthesis.

i.          When plants perform photosynthesis, they produce carbohydrates. These carbohydrates are used for their growth and energy.


ii.          The unutilized carbohydrates are stored in the form of starch.


iii.         While performing experiments on photosynthesis, it is necessary that this stored starch is completely used up.


iv.        When the plant is kept in dark, it utilizes this stored starch and becomes destarched.



v.         The factors essential for photosynthesis such as carbon dioxide, sunlight and chlorophyll can be studied with such destarched plant. Thus, the plants are kept in dark before determining the factors essential for photosynthesis.